26 Years old Maya, a gifted but cold young woman who made a name for herself as the youngest award winning trader ever, is new in the building. A very high-end residence of 42 floors called The Medallion.Luxurious but above all very private, hence the select group of owners living there (political figures, famous lawyers, tycoons etc.. ) Unfortunately she didn't came here to enjoy the premises, a workaholic such as herself doesn't sleep much anyway. But when she end up being neighbour with a more than gorgeous movie star and his very attractive, and very high-end lawyer of a friend, she's in for a treat! Will Maya be able to get her cold facade to melt and stop living her life like she had to go from point A to B ? Furthermore more so when those 2 completely opposite guys start to fall for this ice princess..Who will she choose ?
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It seems the Great uncle Qrow has a daughter who also wanting to be a huntresse as well.let's see what happens to his daughter named Stella Brawnwen
When 22-year-old Aurelia Linamer is suddenly tasked with saving the kingdom alongside the royal heir, she realizes the quest is anything but straightforward. Hidden beneath the surface are layers of deception and untold secrets. Can Aurelia navigate this maze of lies and shadows to save herself and uncover the truth?
Thoughts that were always left unspoken haunts me 'til this day.Published: 12/19/2021
Co-Immunoprecipitation (Co-IP) was developed from the immunoprecipitation technique with which Co-IP shares the fundamental principle of the specific antigen-antibody reaction. Immunoprecipitation(IP) technique is used for isolate individual protein. Using an antibody that is specific for a particular protein, the target protein could be isolated out from a crude lysate of a plant or animal tissue or other biological regent.Immunoprecipitation of intact protein complexes is known as co-IP, which could pull the entire protein complex out of solution and thereby identify unknown members of the complex. Co-IP is a powerful technique that is used regularly by molecular biologists to analyze protein-protein interactions.Prepare lysate form cells or tissue samples which express the target protein is the first step of the co-IP. In order to preserve the intact of protein-protein interactions, the lysis buffers should use non-ionic detergents (e.g., NP-40, Triton X-100). Then the target proteins are captured by specific antibodies from total lysate. The resultant immunocomplexes (composed of antibody, protein of interest (antigen), and antigen-associated proteins) can be precipitated using a resin (e.g., agarose, sepharose, or magnetic beads) that is conjugated with IgG-binding Protein A/G. The third step is washes. Irrelevant, non-binding proteins, antigens and any proteins that are bound are eluted by series of washes. Then, the bound proteins which eluted are analyzed by SDS-PAGE/immunoblotting and/or mass spectrometry.Source: https://goo.gl/2PwKzu
❝ The legal age for wizards is seventeen, Tuney! And by the time I graduate Hogwarts I'll be eighteen, anyway! When that happens, I'll take Harry off your hands. It's the least you could do, considering Lily was your sister, too. ❞ Or, in which a girl who's already lost so much can't stand to lose everything else.[Work In Progress][Cross Posted on AO3]
Knife in your hand. The still air in the hall sends chills down your spine. You've been here many times before, why does it feel different now? I've been here about 50 times... the same faces, the same bloodshed, the same timelines all over again. But this time, it... this. This doesn't feel right.There he is, as always."Let's just get on with it. You're not gonna keep this going as long as I'm here, Demon."And all the same attacks, over and over and over.And over.And over.But you always managed to kill him in even less turns as the timelines went by."*SLASH*"It has been done. Again. You didn't even feel pleasure from this anymore. You changed."And there you go, *cough* you'll reset again. You'll kill again. Y/n, you used to be so different on the FIRST *cough* timeline. What happened?" He managed to say, still gripping tightly his wound."Easy. I became a BETTER person." I chuckled evilly, moving onto the Throneroom, killing Asgore and into the dark abyss where I always press the same button."WAIT! Before you press it, please heed my words!!" A voice. A different one from my ultimate bestie, Chara."Who are you, and what are you talking about?" I looked around. No one.(Who was it? Find out in 1!) -Hallo! A new book, as promised. In chapter 12 or 13, Im gonna play a song related to the book title, for which Caleb Hyles collabed with Jonathan Young, hence the name Start a new again. Part of the song. I bet you already know what the song is. Just wait. OK baiBTW, this is my cover art. You can find it in My Artbook, and you can most definitely use it! Just PM me and mention me about the art.Start date: Nov. 27, 2016End date: January 15, 2017 (wow, I wrote this book in the midst of 2 months or lesser)
⭐시알리스파는곳『ØIØ↔75Ø4↔6Ø45』비아그라구매◈️조루방지제품판매처❤️★정품비아그라&시알리스★⭐#88정퀵배송,#마시는비아그라복제약구입방법,#남성성기단련기구100mg처방⭐시알리스파는곳『ØIØ↔75Ø4↔6Ø45비아그라구매◈️발기부전영양제처방전필요없는❤️★정품비아그라&시알리스★⭐#구구정퀵구입,#신기환파는곳,#발기부전시알리스판매점,#진시환약국판매가격,#남성단련용품종류,#정품비아그라가격,#발기부전치료제직거래,#시알리스퀵,#골드비아그라가격,#남성단련용품퀵배송가격,#필름형비아그라사는곳,⭐시알리스파는곳『ØIØ↔75Ø4↔6Ø45』비아그라구매◈️신기환구매후기❤️★정품비아그라&시알리스★⭐#비아그라파는곳,#프로코밀구매후기,#정품시알리스100mg약국가격,#조루지연크림구매후기,#씨알리스처방전없이구입,#킹통파는곳,#마시는시알리스퀵배송가격,#비닉스복제약구입방법,#약국용비아그라당일구입,#칙칙이가격,#조루치료제종류,⭐시알리스파는곳[ØIØ↔75Ø4↔6Ø45]비아그라구매◈️레비트라복제약가격❤️★정품비아그라&시알리스★⭐#천연비아그라가격,#남성성기단련기구가격,#약국용비아그라당일구매,#캡슐비아그라처방전필요없는,#남자강직도영양제퀵,#남성발기제품처방없이구매,#약국용시알리스효능,#먹는시알리스퀵배송,#vinix직거래,#센트립판매점,#필름비아그라당일구매⭐시알리스파는곳『010↔7504↔6Ø45』비아그라구매◈️a 발기부전 치료제 구매,비뇨기과 비아그라,비아그라약국처방전,핀페시아,카마그라,카마그라 직구,센포스,비달리스타,독일정품프로코밀 후기,시알리스 처방,정품비아그라 성분,발기부전증상,발기부