After the events of Doom of the Universe there are more threats for The Doom Slayer and Samus to take care of. The best part is that they can spend more time with each other and develop their relationship further.
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Hi Friends! This short was a submission for "The One that Got Away" to a paid Wattpad Anthology in 2020. It wasn't selected, but I still want to share it with you. This story was available on my Patreon in 2020. For more shorts/ "before-the-end-of-the-month" short stories, you can find them on my Patreon! https://www.patreon.com/mayenwritesI appreciate all the support! Enjoy!
When 22-year-old Aurelia Linamer is suddenly tasked with saving the kingdom alongside the royal heir, she realizes the quest is anything but straightforward. Hidden beneath the surface are layers of deception and untold secrets. Can Aurelia navigate this maze of lies and shadows to save herself and uncover the truth?
•|Where our protagonist seeks to discover the truths of Brawl park.|•"I'm sorry,But I need to know the truth""in good way....or in bad way...".....⚠️English is not my first language! If you see something spelled wrong, let me know!⚠️All copyrights go to Brawl Stars, Supercell
Co-Immunoprecipitation (Co-IP) was developed from the immunoprecipitation technique with which Co-IP shares the fundamental principle of the specific antigen-antibody reaction. Immunoprecipitation(IP) technique is used for isolate individual protein. Using an antibody that is specific for a particular protein, the target protein could be isolated out from a crude lysate of a plant or animal tissue or other biological regent.Immunoprecipitation of intact protein complexes is known as co-IP, which could pull the entire protein complex out of solution and thereby identify unknown members of the complex. Co-IP is a powerful technique that is used regularly by molecular biologists to analyze protein-protein interactions.Prepare lysate form cells or tissue samples which express the target protein is the first step of the co-IP. In order to preserve the intact of protein-protein interactions, the lysis buffers should use non-ionic detergents (e.g., NP-40, Triton X-100). Then the target proteins are captured by specific antibodies from total lysate. The resultant immunocomplexes (composed of antibody, protein of interest (antigen), and antigen-associated proteins) can be precipitated using a resin (e.g., agarose, sepharose, or magnetic beads) that is conjugated with IgG-binding Protein A/G. The third step is washes. Irrelevant, non-binding proteins, antigens and any proteins that are bound are eluted by series of washes. Then, the bound proteins which eluted are analyzed by SDS-PAGE/immunoblotting and/or mass spectrometry.Source: https://goo.gl/2PwKzu
"You can't repair seven years with two seconds."~^~it has been seven years of Maci struggling to get through the tears. Seven years to try and forgive and forget. To try and just get over him. It just ended in her shoving all her feelings down to leave hate and anger at even the thought of Bentley. The real feelings were just barely under the surface. It seemed that just the day after she had decided she was over it he waltzed into her kitchen. And he didn't even fit his name anymore. He traded his Superman cape in for a leather jacket and his legos in for gauges. She was almost positive that a tattoo or many littered his body. Bentley. That was not Bentley. But as soon as he entered a room Maci knew exactly who it was.
Life and death are one in the same. One gives life, while the other retrieves the life after it's death. Some believe that after death that is the end, that they either go to heaven, hell or end up as a wondering soul on earth.Such as the case of her. Dying at sixteen, she believed that she would move onto the afterlife and stay there until the end of time. But she never would have expected that after dying, she didn't stay dead.There should have only been one child, a son. Should have. But, in fact there was an unexpected variable, the Itadori family had two children. A son Itadori Yuuji, and the youngest Itadori Shina.In this life, she will do everything in her power to save and help her older brother Yuuji. No matter what.MAY CONTAIN SOME SPOILERS OF THE MANGA IN THE LATER CHAPTERS, WHICH I WILL INFORM YOU BEFORE SUCH CHAPTERS START.